Fat is a connective tissue derived from the mesoderm. The fat tissue within the human body is also called the “adipose organ”. Mainly, fat is located within the subcutaneous regions, underneath the skin. Other locations of fat tissue are found within the abdominal cavity (around the intestines and organs). Fat or adipose tissue has important functions within the body and secretes molecules and hormones that are crucial for a good functioning of the human body. It is richly vascularized and a very dynamic tissue. Fat consists of two compartments or fractions: the adipocytes or fat cells and the stomal-vascular fraction (SVF). The SVF contains a heterogenous population of cells and stem cells, the so-called adipose derived stem cells (ADSC). Those stem cells can be used for tissue engineering applications or regenerative medicine applications. The concentration of stem cells within adipose tissue is far higher compared to bone marrow.